#I-11


3D Quantitative Phase Imaging: the needs
and new concepts

Małgorzata KUJAWIŃSKA1, Chao ZUO2, Wojciech KRAUZE1, Maciej TRUSIAK1

1Warsaw University of Technology, Institute of Micromechanics and Photonics, 8 Sw. A. Boboli St., 02-525 Warsaw
2Smart Computational Imaging Laboratory (SCILab), Nanjing University of Science and Technology,
Nanjing, Jiangsu 210094, China

Three-dimensional quantitative phase imaging (3D QPI) delivers volumetric information about the refractive index distribution within microobjects. Such imaging and measurement capabilities are of great interest for technical and biomedical applications, especially as they are complemented by high spatial resolution and basic, label-free sample preparation [1]. However 3D QPI techniques have still several limitations, which restrict their applicability [2]. The most significant problems are connected with:


  • assuring comparable, fully quantitative results delivered by different 3D QPI systems (metrological assessment) including high accuracy data in full measurement volume;
  • achieving high throughput of the system i.e. delivering subcellular imaging resolution and depth sectioning in large field of view;
  • measurements of scattering objects i.e. thicker tissue slices, organoids, small animals;
  • extending 3D QPI applications from in vitro (transmissive mode) to in vivo (reflectance mode) measurements.


The new concepts allowing to partly overcome these constrains will be presented. They include development of metrologically accepted phantoms for validation of 3D QPI systems for both weakly and strongly scattering objects [3,4], application of AI based methods and combining the interferometric (holographic tomography, HT) and non-interferometric (ptychography) QPI techniques to increase the throughput of 3D QPI systems [4] and finally combining concepts of optical diffraction tomography and optical coherence tomography to move towards in vivo measurements [6].

Funding: National Science Centre, Poland (2023/48/Q/ST7/00172), National Natural Science Foundation of China (62361136588), Polish Ministry of Education and Science (Polish Metrology, PM/SP/0079/2021/1) and Warsaw University of Technology under the program Excellence Initiative: Research University (IDUB).



Literature:
[1] Y. Park, C. Depeursinge, G. Popescu, Quantitative phase imaging in biomedicine, Nature Photonics 12 (10) (2018) pp. 578–589.
[2] V. Balasubramani et al., Roadmap on Digital Holography-Based Quantitative Phase Imaging, J. Imaging, 7 (2021), 252
[3] M. Ziemczonok, A. Kuś, M.Kujawińska, Optical diffraction tomography meets metrology—Measurement accuracy on cellular and subcellular level, Measurement, 195 (2022), 111106,
[4] W. Krauze, et al., 3D scattering microphantom sample to assess quantitative accuracy in tomographic phase microscopy techniques, Scientific Reports 12 (2022)
[5] C. Zuo, J. Sun, J. Li, A. Asundi, Q. Chen, Wide-field high-resolution 3D microscopy with Fourier ptychographic diffraction tomography, Opt. and Lasers in Eng.,128 (2020), 106003
[6] W. Krauze, M. Mazur, A.Kuś, qtOCT: quantitative transmission optical coherence tomography, arXiv preprint arXiv:2405.14315 (2024)



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