Krzysztof Banecki
supervisor: Dariusz Plewczyński
The main goal of our project was to create a complete pipeline for the conversion of the microscopic images of the nuclei of a particular cells into their 3-dimensional chromatin models. In our project we used the images of the nuclei of the flowering plant Arabidopsis Thaliana. The local packing of chromatin is consistent with the behavior of a fractal globule with a distinct chromosomal territories which we sought to recreate in our modelling.
Stages of chromatin modelling.
(A) Input microscopic image.
(B) Critical points estimated as local maxima.
(C) Delaunay triangulation graph created on those maxima. Red color signifies larger edge weights.
(D) Critical points divided into chromosomal territories by the graph partitioning algorithm.
(E) Final chromosomal paths smoothed by the splines.